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In contrast, many such situations have been reported in various mutant lines [ 54].
The various mutant lines as homozygotes have been reported over time both as conferring embryo lethality and as displaying no obvious phenotype.
Total lipid content in seeds of Col-0, GmMYB73-overexpressing lines (OE-1, -2, -5, -7 and -10), and various mutant lines was measured.
The morphology of leaf starch granules isolated from wild type and the various mutant lines was examined by scanning electron microscopy.
Figure 7 shows that the levels of most other proteins we examined do not appear to be significantly perturbed in the various mutant lines, with the exception of the D1 protein of PSII, which surprisingly was drastically reduced in amount in TAG-11 and the svr3 single mutants.
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Incubation at 36°C was also used to provide conditions by which the leaf fatty acid profiles would be most clearly delineated among the various Arabidopsis mutant lines as well as to establish the magnitude of the alterations in the fatty acid composition in response to elevated temperature.
Further studies using various growth conditions as well as various cultivars and mutant lines maybe necessary to clarify the machinery of phase change.
Therefore, with viral vectors, specific gene manipulation becomes possible in various normal and mutant lines of rats, mice and probably many other species.
In addition, the functional specificity of the LRR-RLK VIII gene was assessed by treatment of the Arabidopsis mutant lines with various stresses such as As, Cu, Cd, Zn, H2O2 and salt stress.
In various mutants and RNAi lines described above, we also observed an increased number of Dpn+ PntP1+ cells (data not shown), which serves as an independent set of marker for type II neuroblasts.
To obtain functional data on the role of PpHsp16.4 during stress conditions, we monitored growth and chlorophyll content of wild type and mutant lines exposed to various stress factors.
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