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Some mutant lines with adequate supply of seeds have already been distributed to a number of sorghum researchers.
Histopathology could reveal early and subtle lesions before they are severe enough to cause detectable phenotypes, and, for some mutant lines, might represent the only detected abnormality (Schofield et al., 2012).
However, the LORCA has disadvantages in that the sensitivity is not the same as micro-pipetting and as a result we may have missed identifying some mutant lines with rigidity phenotypes.
However, in some mutant lines (e.g. maize lpa2-1), the rise in Pi can be accompanied by an accumulation of other Ins phosphates (Ins(1,2,4,5,6 P5; Ins(1,4,5,6 P4; and Ins(1,2,6 P3) [ 39].
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By the 5th day, some intolerant mutant lines began to die, while the tolerant lines by the 11th day.
Indeed, there are some APC mutant lines which allow βcatenin phosphorylation but are defective in ubiquitination (Yang et al., 2006 JBC).
Building on this proof-of-concept, the following step was to find a way to generate and maintain under collection some putative mutant lines that could then be tested for a phenotype of interest.
In some cases, this includes mutant lines in which individual proteins are absent.
Wild type retinas (Fig. 2A) stained for F-actin to highlight photoreceptor rhabodomeres have 94 µm thick retinas whereas Dg mutants have retinas that were approximately 1/3 thinner (Fig. 2B) with some variance among the mutant lines (Fig. 2C).
While it is largely held that each SS isoform has a unique role in starch biosynthesis, studies of some SS transgenic and mutant lines suggest that there may be functional overlap between isoforms [ 11- 13].
Note that although some mutant variant lines displayed clearly visible truncated C-terminal fragments (∼25 kDa) that have been previously shown to be the result of caspase activation (reviewed by Baloh, 2011), some lines only showed a modest or barely detectable truncation (Fig. 1Y).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com