Exact(4)
Additionally, the zebrafish model offers a number of technical advantages including ease and cost of maintenance, rapid development, high fecundity, optical transparency of embryos supporting phenotypic screening, existence of many mutant strains, or amenability for both forward and reverse genetics.
Interestingly, in many mutant strains we observed substantial production of nuclear 5S rRNA and detected some minor amount of newly synthesized 5S rRNA in the cytoplasm (Fig. 5, lanes 8,10,12,14,26,28,30,32), leaving open the possibility that in yeast 5S rRNA containing particles can translocate to the cytoplasm independently of most of the residual LSU components (see also discussion in [51]).
Many mutant strains are of the same phenotype and have to be kept in separate tanks requiring large rearing facilities and substantial financial support (Sire et al., 2000).
On the other hand, even mutants strongly deficient in in vitro proliferation, such as ckb2Δ, were not necessarily reduced in virulence, and many mutant strains with low fly and mice virulence were unchanged in their in vitro growth (supplementary material Fig. S2).
Similar(4)
The laboratory maintains breeding colonies of many mutant mice strains and can generate rarer strains from a library of frozen embryos.
Many mutant zebrafish strains are used in experimental settings so it was important to evaluate VIE tagging in mutant fish.
However, there needs to be a thorough examination, by Muslims everywhere, of why it is that the faith they love breeds so many violent mutant strains.
There are also many examples of mutant strains expressing virtually identical phenotypes as their wild-type controls, causing concern regarding the appropriateness of these models for the study of physiological processes.
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