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ESC-like colonies were visible under these conditions and emerged as early as 7 21 days post-nucleofection, and manually picked for expansion and further characterization.
Resulting iPS cell colonies were either manually picked for iPS cell line derivation (∼10 12 days after infection), or stained for Nanog as described (∼14 20 days after infection, to enable iPS cell colony formation from keratinocyte controls).
Positive transformants were manually picked for sequencing.
In a single reprogramming experiment several colonies exhibiting hESC-like morphologies were identified and manually picked for expansion and characterization.
After selection, drug-resistant colonies were manually picked for further expansion and screening by genomic PCR and Southern blot.
Similar(55)
Arrival times for these events were manually picked from seismograms at the temporary and permanent stations in the study area.
Particles were first manually picked from 20 micrographs and class averaged using RELION1.3 (Scheres, 2012).
Leaves were manually picked from stems.
We therefore generated clean J2 and dauer cultures, as well as manually picked adults for quantification of expression levels (see Methods ).
Colonies were manually picked up for PCR amplification with M13 and T7 universal vector primers and subsequent sequencing with poly-T primer on an ABI Prism 3,700 sequencer (Applied Biosystems).
The correlation measurements were conducted using 1.2 s window lengths beginning 0.6 s before the manually picked arrival time for 1 10 Hz filtered waveforms.
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