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A total of 550,000 particles (window size 144 × 144) were automatically picked from 2,100 micrographs.
By default, 90% similarity is the cut-off above which the first concept on the retrieved list is automatically picked as the match for the data value and stored in the system.
Peaks above a manually set threshold level were automatically picked by the DOSY Toolbox software for DOSY processing.
For SypHy data, puncta were automatically picked using edge detection on thresholded images in MATLAB and average normalized fluorescence for each region of interest calculated over time.
After CTF determination and correction (performed as for the LdcI-LARA dataset), particles were automatically picked using the Fast Projection Matching algorithm with projections of the cryoET reconstruction as a template.
For three-dimensional (3D) reconstruction of MG23 structures, particle images for the large bowl-shaped and asymmetric small structures were automatically picked up from the electron micrographs.
Automatic structure calculation was conducted using, in addition to the information from the manual calculation, automatically picked peak lists from the CHHC, PAR, PDSD, and two PAIN spectra (Table S2) for iterative assignment by CYANA (Table S2).
"But you play in the first team on merit and because you've played for Nigeria doesn't mean you automatically get picked for the first team". Gabriel could face being disciplined for his outburst.
Automatically picked particles were manually verified using a correspondence analysis based method (Shaikh et al., 2008b).
"A bag left unattended would be automatically picked up by the system".
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