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Tumour and normal brain regions of interest manually drawn using Analyze® software were applied to dynamic PET images to obtain time versus radioactivity curves (TACs), which were corrected for radioactive decay and normalised for injected radioactivity and patient's body weight.

The myocardial slices were weighted and further incubated at 37°C in 1% TTC for 20 min. To avoid any issue of contrast lost or chromatographic change that can occur during digitalization, the boundaries between the infarcted and viable myocardium regions were manually drawn using a binocular magnifier on a transparent sheet directly set in contact with the heart slices.

The neuronal processes and growth cones of all βIII-tubulin positive cells were manually drawn using a computer mouse.

ROIs, chosen due to their predilection for damage post TBI, were manually drawn using Analyze 7.0 (http://www.mayo.edu/bir) in MNI125 space using Colin27 [17] as a high resolution, high signal-to-noise template, and included the corpus callosum (genu and splenium), thalamus, midbrain, pons, cerebellar peduncles, and cerebellar cortex (Figure 1).

To assess LV and RV systolic function, endocardial contours were manually drawn, using MASS software (Medis, Leiden, The Netherlands).

Similar(55)

The resulting tree with Maximum Likelihood analysis branch lengths was drawn using iTOL version 1.8.1 http://itol.embl.de/[ 41], and consensus bootstrap values were added manually.

Conventionally, the staff (1) manually draws [18F]fluoride using a syringe, (2) assays the manually drawn amount using a dose calibrator, and (3) iterates between drawing and assaying [18F]fluoride until the desired amount is collected.

This is explained in detail in Sørensen et al., 2008; briefly, the myelin sheaths were manually drawn over using Adobe Photoshop software, and the percentage of myelin calculated using the area obtained for the highlighted myelin divided by the area obtained for neurite density.

On each section, the outlines of the kidney are manually drawn by using the computer mouse.

Regions of interest were defined by manually drawing circles using the registered MR images as additional reference.

The boundary between white matter and all ventromedial non-neocortical structures was manually drawn making use of a brain atlas [46].

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