Sentence examples for manually prepared using from inspiring English sources

The reaction mixtures were prepared in triplicate in two 384-well plates, A and B. For patient samples n. 104 to 113, real-time PCR reactions were manually prepared using TaqMan® individual MicroRNA Assays (Applied Biosystems) for miR-127 (P/N 4373147), miR-154 (P/N 4373270), and miR-16 (P/N 4373121).

Other corpora exist annotated with protein residue information either manually annotated or prepared using automatic methods.

Models were evaluated using PROSA (https://prosa.services.came.sbg.ac.at/prosa.php) manually inspected, analyzed, and figures were prepared using Pymol (http://pymol.sourceforge.net).net

It is a 5-step procedure which is carried out by selecting the 3D optimized molecules, prepared using Ligprep and manually entering their activity values (pIC50).

Templates for generating dsRNA were prepared using the DRSC or manually designed primer pairs and a 2-step PCR approach.

A multiple alignment of the N-terminal-most domain of the SGCs and its bacterial homologs was prepared using the T_coffee program and adjusted manually based on the PSI-BLAST HSPs (Fig. 1).

Multiple sequence alignments were prepared using CLUSTAL X version 1.82 [ 26], and manually edited in the GeneDoc Alignment editor [ 27].

Multiple sequence alignments and bootstrapped Neighbour-Joining rooted trees were prepared using ClustalX (v. 1.83) [ 46]; alignments were manually edited with Genedoc to remove the alternative spliced exons and visualized with TreeView32.

Multiple sequence alignments of the inferred amino acid sequences were prepared using Multalin [ 72] and ClustalX1.81 [ 73] and manually refined and annotated within BioEdit [ 74] and MacClade4.06 [ 75].

Orthognathic surgery is indicated for the treatment of significant skeletal malocclusion and is normally prepared using conventional face bows combined with two-dimensional cephalometric analysis and manually made splints.

Preintervention IVUS was performed without complications in 46 patients with AMI using manually prepared contrast medium.