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Primers were manually generated using sequences available in GenBank (Additional file 4).
A basic network model of the RANKL pathway applying the above criteria was manually generated using PathVisio software.
However there are published cell cycle models not yet implemented in SBML: for this reason some SBML models included in the database are manually generated using the JigCell Model Builder software [ 34], a model editor which allows the construction of biochemical reaction networks in SBML format, and are validated using the Systems Biology Workbench SBML validator.
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Manually annotated sequences have been generated using in-house developed software (55) in accordance with the manual annotation guidelines (56).
Since consensus sequences inferred by AbundantOTU are often shorter than the targeted amplicon, we manually examined multiple sequence alignments, generated using MUSCLE [ 30], of reads from each OTU to identify a reference sequence spanning the entire V1-V3 region.
As a standard method, parametric images of binding potential (( {BP}_{mathsf{ND}}^{ast } )) were initially generated using reference tissue manually defined on the cerebellar cortex.
Sequence alignments were generated using clustalW and manually corrected using BioEdit.
For projects such as Plasmodium falciparum that are being manually curated, gene structure annotation is generated using several different large data sets and methods.
For each time-lapse movie, mitochondria or autophagic vacuoles were manually tracked and transport parameters were generated using the ImageJ software plug-in Multiple Kymograph, submitted by J. Rietdorf and A. Seitz (European Molecular Biology Laboratory, Heidelberg, Germany).
Multiple sequence alignments were generated using MUSCLE and trimmed manually using Mesquite v2.75.
Results generated using PEAKS® Studio were manually curated against the MASCOT search engine results.
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