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To prevent acidification of the culture media and maintain tissue viability, intestinal IVOCs require a hyperoxic gas environment [4 6].

Biopsy specimens were stored on ice in high-potassium cardioplegic solution (5% glucose, 68.6 mM mannitol, 12.5 meq potassium chloride, 12.5 meq sodium bicarbonate, 10 units/mL heparin) to maintain tissue viability during transport.

Mammary and liver tissues were kept in 25 mmol/L Tris, 0.25 mol/L sucrose, 1 mmol/L EDTA (pH 7.3) on ice, and adipose tissue was kept in saline at 37°C to maintain tissue viability during the time between tissue removal and incubation for metabolic analysis.

Therefore, the scavenging of oxygen free radicals induced immediately after ischemia-reperfusion may not only maintain tissue viability but also enable sufficient contractile function of skeletal muscle.

In the neonatal phase in mammals, apoptosis is coupled to autophagy as a means to maintain tissue viability and energy homeostasis in developing tissues.

In addition, the therapeutic potential of DDFPe has been applied to supplement oxygen delivery and maintain tissue viability in severely anemic rats and to reduce infarct size in a rabbit model of ischemic stroke [ 8– 10].

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3D bioprinting of vasculature network is particularly discussed for its foremost significance in maintaining tissue viability and promoting functional maturation.

Furthermore, maintaining tissue viability and stability is also more difficult than for cell culture systems, where more optimal imaging conditions can easily be achieved.

However, vascular tissue culture has been used to investigate DNA and protein synthesis in many different vessel types [ 56– 59], maintaining tissue viability, contractility, and relaxation for up to 5 days [ 59, 60].

The loop was glued (3M Vetbond TissueAdhesive, St . Paul MN, USA) on a heated metallic block and sliced so that the mucosa could be carefully pressed to a fixed microscopic cover slip to dampen movement artefacts, yet maintained tissue viability (Fig.  1).

The ex vivo bioreactor simulates the physiological conditions and helps to maintain the tissue viability, but as consequences of inherent tissue sensitivity to hypoxia the endothelium is denuded [ 16].

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