3D bioprinting of vasculature network is particularly discussed for its foremost significance in maintaining tissue viability and promoting functional maturation.
To prevent acidification of the culture media and maintain tissue viability, intestinal IVOCs require a hyperoxic gas environment [4 6].
Biopsy specimens were stored on ice in high-potassium cardioplegic solution (5% glucose, 68.6 mM mannitol, 12.5 meq potassium chloride, 12.5 meq sodium bicarbonate, 10 units/mL heparin) to maintain tissue viability during transport.
Mammary and liver tissues were kept in 25 mmol/L Tris, 0.25 mol/L sucrose, 1 mmol/L EDTA (pH 7.3) on ice, and adipose tissue was kept in saline at 37°C to maintain tissue viability during the time between tissue removal and incubation for metabolic analysis.
In the neonatal phase in mammals, apoptosis is coupled to autophagy as a means to maintain tissue viability and energy homeostasis in developing tissues.
Furthermore, maintaining tissue viability and stability is also more difficult than for cell culture systems, where more optimal imaging conditions can easily be achieved.
Therefore, the scavenging of oxygen free radicals induced immediately after ischemia-reperfusion may not only maintain tissue viability but also enable sufficient contractile function of skeletal muscle.
However, vascular tissue culture has been used to investigate DNA and protein synthesis in many different vessel types [ 56– 59], maintaining tissue viability, contractility, and relaxation for up to 5 days [ 59, 60].
In addition, the therapeutic potential of DDFPe has been applied to supplement oxygen delivery and maintain tissue viability in severely anemic rats and to reduce infarct size in a rabbit model of ischemic stroke [ 8– 10].
The endometrioma observed in this dog was highly vascularized, which was important to maintain tissue viability as areas of necrosis were not observed despite the large size of the mass.
