Delivery of nutrients and oxygen within three-dimensional (3D) tissue constructs is important to maintain cell viability.
To maintain cell viability and enhance antiviral and antitumor effects, NK cells are frequently treated with cytokines.
The biological 3D construct could maintain cell viability, thereby facilitating cell spreading and matrix formation.
In addition, adequate nutrient supply to the capsules is required to maintain cell viability.
Hepatocyte organoids have an in vivo-like cell morphology and maintain cell viability and function in vitro.
To reduce the overall analysis time during live cell SECM measurements and maintain cell viability, the microelectrode scan rate can be increased.
To maintain cell viability in response to glucose deprivation, Akt-transformed cells must begin to catabolize fatty acids to provide mitochondria with sufficient substrates to maintain oxidative phosphorylation and cell survival.
Collectively, the data indicate that ECM coatings (i.e., bECM and MaxGel) are not required to maintain cell viability, do not alter the proliferative capacity of glial cells, and do not affect the astrocytic phenotype in long-term cultures.
Further development of this cryopreservation method is necessary to minimize the alteration in material property and maintain cell viability of the constructs.
A bioreactor must support 3D tissue development, maintain cell viability and function within a tissue, and provide appropriate molecular and physical cues.
These complex structures, including toroids, honeycombs, and loop-ended dogbones, bypass the critical diffusion distance required to maintain cell viability in culture over time.
