Drops were dispensed in 96-well plates by a Mosquito liquid handling system (TTP Labtech).
A robotic liquid handling system was employed to carry out a parallel batch screen on a displacer library made up of analogous compounds.
This approach comprises the combination of a Biomek FX liquid handling system with 96-channel pipetting capability and a custom-designed 96-well format on-line incubator with efficient thermal conductivity.
The adsorption isotherms on aluminum hydroxide and aluminum phosphate of two model proteins, p-casein, and bovine serum albumin, were evaluated using a liquid handling system, which permitted rapid sample preparation in a small volume without nonspecific adsorption.
A library of methyl glycosides consisting of ten common monosaccharide substrates, along with oligosaccharide substrates, was screened with the artificial glycosidase for hydrolytic activity in a high-throughput format with a robotic liquid handling system.
All liquid handling for the 96-well microplates was performed using a Biomek® NX Span-8 liquid handling system.
All reaction set-ups were performed using the epMotion® 5075LH automated liquid handling system (Eppendorf, Hamburg, Germany).
Per 96 well plate, 1160 µl buffer I was mixed with 580 µl buffer H to make buffer J. Using a Biomek 3000 liquid handling system, 85 µl of buffer G was added to each well.
We utilized 96-well optical-bottomed plates and a liquid handling system to ensure the exact reaction time for all of the wells in each step in the staining procedure.
Image cytometry techniques can accommodate most staining techniques developed for flow cytometry, and are amenable to automatic sample preparation because each solution-phase reaction can be carried out in place, where cells are fixed on, using a liquid handling system.
Assays for high throughput screening were prepared using a Beckman Coulter Biomek 3000 liquid handling system equipped with a 200 µl single channel and 20 µl 8 channel pipette head.
