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All the liquid handling steps in the HTS assays were performed using a Freedom Evo 75 robotic workstation equipped with on-deck Te-Shake orbital mixing platform and 8 plus 1 Access eight-channel liquid handling arm (Tecan Group Ltd ,Durham, NC).
This model was equipped with a four-syringe LIHA (liquid handling arm) and a 96-well MCA.
This robot has one liquid handling arm and one arm for lifting plates, but no plate heaters or shakers.
The liquid handling arm was equipped with 1 mL syringes and was used with disposable tips only to minimize cross contamination.
Pretreatment, hydrolysis and sugar determination are performed automatically by a robotic platform (Tecan Evo 200; Tecan Group Ltd. Männedorf, Switzerland), equipped with a liquid handling arm and a robotic arm for the movement of plates.
A Tecan robot (Freedom Evo 150, Tecan Group Ltd., Männedorf, Switzerland) equipped with a liquid handling arm (1) was used to accurately dispense the nutrient screen matrix stock solutions into 1,728 wells.
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PrePCR processing was performed using a Beckman FxP dual arm liquid handling platform.
Liquid transfer was carried out using a Tecan Freedom Evo™ liquid handling robot equipped with a multi-channel arm (Tecan Group Ltd ,Mannedorf, Switzerland).
All PCR and sequencing reactions were set up on a Tecan Genesis RSP200 liquid handling workstation, with a robotic and an 8-channel pipetting arm, an integrated 96-channel pipetting head (TEMO96, Tecan), and four integrated dual-384 well PCR blocks (Applied Biosystems).
The liquid methanol was injected by patented Cheminert® M Series liquid handling pump.
The long hood features a WellMate liquid handling instrument.
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