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For purification of the PCR products, quantification of amplicons and equimolar pooling, commonly referred to as library preparation, was done on a Microlab STAR liquid handling robot (Hamilton Robotics, Martinsried, Germany).
The matrix-siRNA samples used for array printing were prepared to U-bottom 384 wells (Ab1055, Abgene) with a Hamilton STAR 96-channel liquid handling robot (Hamilton Robotics) in sterile conditions.
Q-PCR experiments were assembled by a liquid handling robot, a CAS-1200 robot (Corbett Robotics, Australia).
The difference between the two robotics platforms is that the BiomekFX liquid handling robot can transfer liquids from one location to another, whereas the Maxwell® 16 transfers the paramagnetic beads – not the liquid.
Into a 96-well plate uniformly filled with rich media (THB), S. mutans bacteria were inoculated into alternating wells using an automated liquid handling robot (a).
Our hardware setup consists of a Tecan Genesis RSP 159 liquid handling robot and a Tecan Ultra II plate reader.
After ligand incubation was complete, cells were fixed and endogenous AR was immunolabeled using the Biomek NX liquid handling robot.
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Builders here say a week of work in Shenzhen is equal to a month in the U.S. "If you're an engineer with an idea and you're waiting five days or two weeks to test it, that's no way of being creative," said Will Canine, co-founder of OpenTrons, a company building an open-source liquid-handling robot in Shenzhen.
Spotting, in principle, could also be automated using a standard liquid-handling robot.
(2003), with modifications to accommodate use of a Perkin Elmer MPII liquid-handling robot.
The automatic library construction using the Agilent Bravo liquid-handling robot contributed to the reproducibility among the samples.
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