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A liquid handling Workstation epMotion 5070 (Eppendorf) was used to optimize RT-PCR assays with small reaction volumes.
The remainder was added by the liquid handling workstation.
All experimental procedures were carried out using a TECAN Freedom Evo II liquid handling workstation.
This system consists of a BioLector integrated into a liquid handling workstation.
Cells were stained using a Beckman Coulter Biomek FX liquid handling workstation with 384-well tip head.
To ensure highly reproducible pipetting, we ran the experiments on a Tecan Freedom Evo II liquid handling workstation.
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Then, 1.25 μl of the forward/reverse primer mix (1 μM stock solution) was added to the 8.75 μl master mix using a Freedom EVO Tecan liquid handling robotic workstation, resulting in a final volume of 10 μl per reaction.
Typical areas include materials handling, workstation design, physical environment and work organization.
Many concrete improvements have resulted in materials handling, workstation design and physical as well as psychosocial environments.
Details of sample handling involved a sample collection map, sample collection formatting and volume, dilution schemes for high concentration samples, choice of biological fluid and evaluating the capabilities of two liquid-handling workstations.
Taken together, these studies suggest that organisms dispensed by automated liquid-handling workstations and cultivated in microtiter plates may provide an economical alternative to molecular and cell-based screens.
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