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Compound solutions were then transferred into the biosensor wells using the on-board liquid handling device, and the cell responses were recorded in real time.
using an acoustic liquid handling device, Echo 550 (Labcyte , Inc.
A Titertek MapC2 liquid handling device was used to dispense 60 μl of Lysis Buffer (Qiagen Buffer P2).
Cell solutions were dispensed using an automated liquid handling device, and cells exposed to drug (at a concentration of 10 μℳ) or control (0.1% DMSO) in growth media for 5 days, 24 h after seeding.
In order (i) to render the FADU assay more convenient and robust, (ii) to increase throughput, and (iii) to reduce the number of cells needed, we have established a modified assay version that is largely automated and is based on the use of a liquid handling device.
The modified and automated version of the FADU assay described in the present paper enables measurement of DNA strand breaks and DNA repair in a very reliable and convenient manner by exploiting the power of a liquid handling device (LHD) in terms of its extremely high level of control of various parameters and the reproducibility of dispensing small volumes.
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Fortuitous parallel developments of automated systems such as liquid handling devices and high-throughput plate readers place genome-wide studies of direct biomedical relevance within immediate reach.
Process-related mishaps in the pipeline would include missed colony picks, blocked capillaries on sequencers, faulty tips on automated liquid handling devices, or lost DNA template during precipitation.
Large-scale parallel selections have been done with microplate technologies, which are of particular interest due to the availability of protocols and automated liquid handling devices [ 7, 8].
The three main methods currently in use for saccharification studies involve (1) utilizing fully automatic weighing apparatus [ 7], (2) drying the material into thin handbills of regular thickness and weight and then punching out discs [ 8] and (3) making slurries of the material in order to allow it to be pipetted with standard liquid handling devices [ 4].
The method is conceived for samples that include an adequate amount and quality of DNA, such as found in fresh clinical and forensic samples and can be conducted by any laboratory in the molecular field without the requirement for automated liquid handling devices.
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