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All experimental tools were developed using the robotic liquid handling station Tecan Freedom Evo 200.
A method to generate phase diagrams in high throughput on an automated liquid handling station in microbatch scale was developed.
Several PEG/phosphate systems varying in PEG molecular weight, pH and NaCl concentration were investigated in an automated small scale screening performed on a liquid handling station.
Densities were measured by a standalone micro fluidic liquid density sensor, which was integrated into a liquid handling station by means of a developed generic Tip2World interface.
Results are presented from the optimal operation of a fully automated robotic liquid handling station where parallel experiments are performed for calibrating a kinetic fermentation model.
Several methods were used throughout the duration of the project: Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich), or in-house developed protocols Chelex-based 'DryRelease', silica-based 'Silitom' [43] and automated DNA extraction protocol on the Biomek FX© liquid handling station using 1.0 µm PALL glass fiber media filter plates [44].
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Full automation was achieved by establishing a method to automate pH and salt concentration adjustment on liquid handling stations.
This combination of liquid handling stations and lab-on-a-chip technology enables fast, fully automated, and highly accurate density measurements.
Moreover, liquid handling stations with multi-channel pipette heads (automated or manually operated) are applied for replicating clones [ 20].
To improve the reproducibility of high-throughput cultivation processes and the comparability between different applied cultures, it is strongly recommended to use automated or manual liquid handling stations or, alternatively, multi-channel pipettes.
DNA extractions were performed with the NucleoSpin96 (Machery-Nagel) kit according to the specification of the manufacturer under automation with a Biomek NX liquid-handling station (Beckman-Coulter) equipped with a filtration manifold as previously described [40], [41].
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