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The brains were removed, fixed in 10% paraformaldehyde overnight, and then cryoprotected in 30% sucrose solution.
The brains were removed, fixed in 10% formalin, frozen to -25°C and cut into 20 30 μm coronal sections.
The brains were removed, fixed in the same solution overnight, and then stored at 4°C in PB containing 30% sucrose.
35 Briefly, following transcardial perfusion with heparinized Phosphate buffered saline (PBS), brains were removed fixed in 4% paraformaldehyde, and sliced coronally at 50 μm.
Brains were removed, fixed in cold 4% neutral buffer paraformaldehyde for 48 h, and cryoprotected by immersion into 20% sucrose phosphate buffer for 48 h at 4°C.
The brains were removed, fixed in 10% formalin, frozen, cut in 60 μm serial coronal sections, stained with Giemsa, and analyzed by light microscopy to confirm the injection sites into the LPBN.
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Upon his death in 1955, Albert Einstein's brain was removed, fixed and photographed from multiple angles.
The brain was removed, fixed in Carnoy's solution, and embedded in paraffin for subsequent histological procedures.
The brain was removed, fixed in 4% PFA overnight, and stored in phosphate buffer at 4°C before histological processing for all brains.
After implantation, the brain was removed, fixed in 4.0% paraformaldehyde in phosphate-buffered saline, and subsequently cryosectioned to view the implant placement.
Brains and stomachs were removed, fixed with 10%% formalin and embedded in paraffin.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com