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Brains were removed, postfixed for ∼8 h in 4% paraformaldehyde, and kept in PBS overnight.
The brains were removed, postfixed in 4% paraformaldehyde and paraffin embedded.
The brains were removed, postfixed in the same solution at 4°C overnight, cryoprotected, frozen, and 30 µm coronal sections were obtained in a cryostat.
Then, the brains were removed, postfixed for 30 min in paraformaldehyde, submerged for 36 h in 20% sucrose at 4°C, and frozen in isopentane (−40°C).
Brains were removed, postfixed in PLP solution overnight at 4°C and paraffin-embedded.
Brains were removed, postfixed, and transferred to 30% sucrose for 4 days.
Similar(34)
The brain was removed, postfixed overnight, cryoproteced with 30% sucrose then sectioned at 100 µm on a freezing sledge microtome.
Subsequently the brain was removed, postfixed for 24 h in 4% paraformaldehyde in PBS, and cryoprotected by immersion in 30% sucrose in PBS for 48 h.
The brains and eyes were removed, postfixed for 1 2 days, and processed for histological examination as described below.
Brains and spinal cords were removed, postfixed overnight and cryoprotected in 30% sucrose in phosphate buffer for 24 h.
Brains and spinal cords were removed, postfixed in the same fixative overnight at 4 °C, and cryoprotected with 20% sucrose.
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