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Mice were then perfused, and brains were dissected, fixed, and sectioned at 40 μm with a sliding microtome 2010 (Leica, Allendale, NJ, USA).
Adult male brains were dissected, fixed, treated with primary and secondary antibodies, and prepared for confocal imaging as described previously [68].
Larval brains were dissected, fixed, and stained as described before (Lee and Luo, 1999).
Adult fly brains were dissected, fixed and stained using standard protocols (Aso et al., 2009).
The brains were dissected, fixed overnight in 10% neutral buffered formalin, and paraffin-embedded using standard techniques.
Mice were anesthetized by isoflurane (Clipper Distributing) and brains were dissected, fixed with 4% PFA at 4°C overnight, and cryoprotected in 20% sucrose in PBS (pH 7.4).
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Primary tumors and whole lung, liver and brain tissues were dissected, fixed in 10% formalin (Sigma), embedded in paraffin, cut into 2 µm sections and stained with hematoxylin and eosin.
Briefly, brains of E18.5 embryos were dissected, fixed in Bouin's solution and embedded in paraffin.
Likewise, the brains of postnatal pups (P7) were dissected, fixed, cryo-protected, and sectioned at 20 µm.
The brains from age-matched female adults were dissected, fixed and observed using confocal microscopy.
The brains were dissected out, fixed by immersion with Bouin fixative for 72 h, embedded in paraffin and serially sectioned.
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