Whole brains were obtained, fixed, embedded and sectioned for two P15.5wk +/tg380a mice and one littermate control.
Whole brains were obtained, fixed, embedded and sectioned for two p70 human PWS-IC/C57 (HsIC/+) mice and two wt littermates (37).
Whole brains were obtained, fixed, embedded and sectioned for two P8.5wk FVB/PWS-IC 35kb deletion (+/PWS-ICdel35kb) mice and one wt littermate (36).
For immunofluorescence staining and imaging of brain metastases, samples from the mice brain were obtained, fixed in 4% paraformaldehyde, dehydrated in alcohol, cleared in xylene, and embedded in paraffin.
Wt male C57BL/6J whole brains for multiple age time points were obtained, fixed and embedded in paraffin and were sectioned (10 µm) for embryonic day 15 (E15), post-natal day 1 (P14, P28, P28 and P70, liver, thymus, kidney and spleen were also taken from a P70 mouse.
In addition to marmoset brains fixed as described above, E13.5 mouse brains and E39 and P1 ferret brains were obtained, PFA fixed and subjected to immunohistochemistry as described previously (Pulvers and Huttner 2009; Fietz et al. 2010; Arai et al. 2011).
Brains were obtained as described earlier and fixed in 10% neutral-buffered formalin.
For embryonic stages E85 and E100, fixed embryonic brains were obtained from the Wisconsin National Primate Center in Madison, WI (E85) and the German Primate Center in Götingen, Germany (E100).
Human brains were obtained 12 24 hrs postmortem.
Formalin fixed paraffin embedded sections (6 μm thick) of rat brain were obtained from Genostaff (Tokyo, Japan).
Lung, nasal turbinate, nasal septum, larynx, trachea, bronchus, tracheobronchial lymph node, nictitating membrane, tonsil, heart, liver, spleen, kidney, pancreas, duodenum, jejunum, colon, adrenal gland, brain, and olfactory bulb samples were obtained, were fixed in formalin, and processed to obtain sections for staining with hematoxylin and eosin.
