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On day 12, 3 mice from each experimental group were euthanized and their ears were removed, fixed in 10% formalin and processed for H&E-stained paraffin sections and for immunohistochemical staining.
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Tumors were removed, fixed and maintained in paraffin blocks.
Kidneys were removed, fixed and embedded in paraffin.
Lungs were removed, fixed in 10%% formalin, and paraffin embedded.
Kidneys were removed, fixed with formaldehyde, and embedded into paraffin.
Inner ears were removed and the round and oval windows were opened to permit flushing of fix through the scalae followed by postfixation overnight at 4°C.
Both ears were removed and weighed.
For the isolation of murine ear fibroblasts (MEFs), mice were sacrificed and ears were removed.
Both ears were removed at the indicated time points and control mice did not have their ears removed.
Two hours after xylene application, the mice were sacrificed and both ears were removed.
The ears were removed from NKB treated, positive control and control groups of mice.
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