Brains of euthanized mice were collected, fixed in 4% paraformaldehyde, paraffin embedded, sectioned, and subjected to hematoxylin and eosin staining.
Tissues from brain, trachea, lungs, mesenteric lymph nodes, intestines (duodenum, jejunum, and ileum), kidneys, liver, pancreas, spleen, and heart were collected, fixed with 10% buffered formalin, and processed for histopathologic examination.
Lungs were collected, fixed and embedded into paraffin.
Tissues were collected, fixed in formalin, and embedded in paraffin.
Pancreatic tissue was collected, fixed, and processed as described above.
After recording, the brains were collected and fixed in 4% formaldehyde in PBS at 4°C for 10 h.
After the Morris water maze, the mice were euthanized under anesthesia, and the brains were collected and fixed in 25% glutaraldehyde for 2 h at 4°C, rinsed with PBS, and soaked in osmium tetroxide.
Briefly, brains were collected at autopsy, fixed in 15% neutral buffered formalin for two weeks, the fixed brain weighed and the volume determined by fluid displacement.
Mice brains were collected after PBS perfusion and fixed with 10% neutral buffered formalin, pH 7.0, for 12 h at 4°C.
Brains were collected and the right brain hemisphere was fixed in 10% buffered neutral formalin (BNF) for 3 4 weeks.
