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Brains were cut using a cryostat at −20°C.
20 µm coronal sections of embryonic and PND0 brains were cut using a cryostat.
The brains were cut using the Leica CM 3050 cryostat machine at 40 micrometers thin slices and stored at 6°C in 0.02 M PBS buffer (pH 7.4).
Mouse brains were cut using a cryostat (Leica CM3050) and every fifth serial coronal section (30 μm) was stained with FJC.
Sections (6 μm) of formalin-fixed brains were cut using an RNase-free blade, mounted and dried overnight at 63°C.
Frozen brains were cut using a Leica CM-3050S cryostat (Leica Microsystems, Buffalo Grove, IL, USA), and 12 μm frontal brain slices of control and EAE mice were mounted on the same electrostatic Superfrost plus slides.
Similar(54)
Coronal sections of whole brain were cut using a vibratome (Campden Instruments, Lafayette, Indiana) up until the level of the suprachiasmatic nucleus.
Coronal sections (40 μm) throughout the brain were cut using a freezing microtome and stored at −20°C in phosphate buffer (pH 7.4) containing 30% glycerol and 30% ethylene glycol.
Coronal sections of brain were cut using a Leica VT 1000S tissue slicer, slices were incubated in oxygenated aCSF containing sucrose for 30 min at 35°C, then for ≥1 h at 22°C, and transferred to an open bath perfusion chamber (Warner Instruments).
Brains were rapidly removed and coronal brain slices (400 μm) were cut using a vibrating microtome (Microslicer DTK 1500, Dousaka EM Co., Kyoto, Japan) in ice-cold cutting solution composed of (in mM) 94 sucrose, 30 NaCl, 4.5 KCl, 1.0 MgCl26 26 NaHCO3, 1.2 NandPO4, and 10 -glucose with pH 7.4.
Four-micrometer-thick sections of paraffin-embedded brain, skin and heart were cut using a microtome and collected on Superfrost Plus glass slides (Thermo Scientific, Waltham, MA, USA), which were then incubated for 30 min at 60°C for adhesion.
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