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Total RNA of the infected FB brains were extracted using Trizol reagent (Invitrogen, Carlsbad, CA).
For estimating the level of brain transcripts, total RNAs from four E18.5 mouse brains were extracted using Trizol (Invitrogen, NY) and RNAeasy kit (Qiagen, CA) and mRNA was amplified and labeled by the method provided by Affymetrix.
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For further analysis RNA from 3.5 4 month old mouse brains was extracted using Trizol (Invitrogen).
RNA from LV-injected brains was extracted using a RNeasy Lipid Tissue kit (Qiagen).
Total RNA from brains was extracted using TRIzol reagent (Gibco-BRL, Life Technologies).
For whole brain (Elav-Gal4 > w ), total RNA from third instar larval brains was extracted using Trizol (Invitrogen) following the manufacturer's instructions.
Total RNA of ITs and brain were extracted using QIAGEN RNeasy Microkit according to the manufacturer's protocol.
The brain was extracted using the Brian Extraction Tool (BET v2.1) in FSL.
Each subject's whole brain was extracted using the Oxford centre for functional MRI of the brain (FMRIB) software library (FSL) [24] brain extraction tool (BET) [25] and the corresponding grey matter probability maps were created using statistical parametric mapping (SPM5) software [26].
Total RNA from brain was extracted using TRIzol reagent (Gibco BRL) according to the manufacturer's instructions.
Total RNA from adult C57BL/6 mouse brain was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
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