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Brains were removed using standard techniques, weighed, and sagittally cut through the corpus callosum and brainstem.
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For experiments that used larvae 4 dpf or beyond, the skin covering the brain was removed using sharpened tungsten needles (under modified ACSF containing 0.02% MS 222) to expose the brain for imaging from the dorsal side.
As soon as possible after expulsion (less than 6 hours), the brain was removed using the standard fetal autopsy procedure (Valdes-Dapena, 1983), embedded as a whole in OCT compound (Sakura), and snap-frozen in a 2-methylbutane on dry ice bath.
The non-brain parts were removed using Brain Extraction (BET) [31].
Non-brain structures were removed using FMRIB's Brain Extraction Tool.
The brain hemispheres were removed using standard techniques, exercising extra care to avoid damaging the brain tissue.
Liver, brain, and heart were removed using scissors and tongs.
These training datasets were used by the algorithms for the segmentation of the subjects intracranial brain volume after the nonbrain structures were removed using the Brain Extraction Tool FSL-BET [ 22].
EEG preprocessing: Eye movement artifacts were removed using Brain Vision Analyzer (Brain Products GmbH) through an independent component analysis (ICA).
The head of each bee was removed using a sterile blade, followed by the frontal section of the head capsule, which was carefully removed to reveal the brain.
Brains were removed and used for histological analysis.
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