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Their brains were imaged using high-resolution diffusion tensor imaging (DTI) on a 7T small-animal magnetic resonance imaging system.
Stained whole brains were imaged using confocal microscopy (LSM 510).
Drosophila brains were imaged using confocal microscopy Nikon AIR confocal unit mounted on a TI2000 inverted microscope (Nikon Corp).
Adult brains were imaged using a Zeiss 510 Confocal Laser Scanning Microscope (Carl Zeiss Microscopy, Jena, Thuringia, Germany).
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After sacrificing the mice, the whole brain was dissected and ultrathin sections (20 µm) of tumor bearing brain were imaged using the same fluorescence imaging system as used for in vivo.
Based on prior studies, we anticipated that the anterior corpus callosum would send projections to the anterior cerebral cortex whereas progressively posterior segments would send projections to more posterior cortex.A postmortem canine brain was imaged using a 7-T MRI system producing 100-μm-isotropic-resolution diffusion-tensor imanalyzedalyzed by tractography.
A 1 mm area of each of the PNGase F negative and positive parts of the brain was imaged using MALDI-MS separately.
Brain sections were imaged using brightfield microscopy, and optical density of striatal staining was determined using ImageJ software.
For quantification of apoE/CAA or apoE/plaque co-staining in 10-month-old 5XFAD/apoEm/4 mice, brain sections were imaged using a Zeiss LSM 5 PASCAL system coupled to a Zeiss Axiovert 200 M confocal microscope.
Brain tissues were imaged using an Olympus Ultra 25X MPE water immersion objective (1.05 NA), with filter set bandwidths optimized for CFP (460 500 nm), YFP (520 560 nm), Texas Red/DsRed (575 630 nm) and Qdot 705/800 (669–800 nm) imaging.
Stained whole organs and sagittal brain sections were imaged using a Leica M216FA stereomicroscope (Leica Microsystems, Buffalo Grove, IL) equipped with a DFC300 FX digital imaging camera (Watson et al., 2008).
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