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Expressions of the four isoforms β2L, β2S, β2S1 and β2S2 in DLPFC of postmortem CON, SCZ and BPD brains were determined using quantitative real-time PCR, and normalized by the geometric mean of the three reference genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin C (UBC) and hydroxymethyl-bilane synthase as described [11].
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WldS protein levels in the brain were determined using quantitative fluorescent western blotting.
The frequencies of IFN-γ-producing CD3+CD4+ and CD3+CD8+ T cells among the CD3+ lymphocytes in various tissues (i.e., ileum, spleen, blood, lung and brain) were determined using intracellular staining and flow cytometry.
Regional distribution of PRRT2, SLC2A1, PNKD, KCN1A, SNAP25 and CACNA1A mRNA expression in the normal human brain was determined using microarray analysis of human post-mortem brain tissue from the UK Human Brain Expression Consortium (Trabzuni et al., 2011).
In a recent study of fragile X mental retardation 1 (Fmr1) knockout mice, the metabolic profile of the fragile X brain was determined using proton high-resolution magic angle spinning nuclear magnetic resonance spectroscopy.
The infarct area and edema volume in seven coronal sections of each brain was determined using the scanned image with a CCD camera (Samsung, Korea) and quantified with Biovis, Expert vision, (India).
ATP levels in dissociated brain cells were determined using the bioluminescent measurement of ATP as published [45].
The Aβ1-40 and 1-42 levels in the supernatants and in mouse brain homogenates were determined using specific sandwich ELISA kits as previously described, and following the manufacturer's instructions [11], [12] (Wako Chemicals, Japan).
Brain eQTLs were determined using methods published previously [ 69].
The levels of DA in the brain tissues were determined using a modified method.
The concentrations of phosphatidylcholine hydroperoxide (PC-OOH) in liver and brain samples were determined using chemiluminescence high-performance liquid chromatography.
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