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Brains were sectioned using a vibrotome with 1 mm spacing.
All brains were sectioned using vibrating microtome at a thickness of 40-μm.
Frozen brains were sectioned using a cryostat (Leica CM3050, Bensheim, Germany) and coronal sections (10 μm) of whole visual cortices were thaw-mounted on adhesive silane-coated slides (Histobond, Marienfeld, Laboratory Glassware, Lauda-Königshofen, Germany).
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Briefly, brains were isolated and slices were sectioned using a McIlwain tissue chopper (350 μm) under sterile conditions (laminar air flow bench).
Fixed brains were sectioned and mounted using an identical method to that described above.
For in situ, brains were sectioned (12 μm) using a cryostat freezing microtome.
Frozen brains were sectioned coronally (100 150 μm) using a cryostat (Leica CM 1900, IL).
Brains were sectioned at 30 µm using a cryostat.
For immunohistochemical procedures (described below), the brains were sectioned at 30 µm using a sliding microtome (AO Scientific Instruments, Buffalo, NY, USA).
Brains were sectioned at 25 μm using a cryostat, stained with cresyl violet, and imaged.
Brains were sectioned (coronal; 50 μm) using a vibrating microtome (VT1000S, Leica Instruments, Nussloch, Germany).
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