Sentence examples for binding affinity has been from inspiring English sources

From the second binding event a binding affinity has been calculated, with a Kd of 0.6 µM.

This use of disordered region to enhance the rate of interaction and uncouple specificity from binding affinity has been observed in the self-assembly processes of viruses and bacterial flagella, specifically during the process of regulating the addition of new components to the growing assembly [33], [34].

Altered binding affinity has been shown to have substantial impacts on the contributions of TFBS [ 35].

However, the typical increase in binding affinity has been only 10-fold using these approaches, which would still not produce binders comparable to the best conventional antibodies [ 9].

Subsequently, several surface-exposed charged amino acids have been mutated to alanine or substituted by reversed charges in human coronin-1B and their F-actin binding affinity has been analyzed ([ 40], Figure 6 red dots, see also Additional file 5).

A preliminary assessment of the apparent K m values of rF6Hb for the flavonol substrate (63 μM) suggests that the binding affinity has been reduced 17-fold compared to the native protein (0.27 μM) [ 3].

To understand this, comprehensive analysis of hydrophobic interactions, hydrogen bonding and binding affinity have been analyzed at the interface of c-Src and c-Abl kinases and 4-amino substituted 1H-pyrazolo [3, 4-d] pyrimidine compounds.

To overcome these limitations, phage antibody libraries were made from immunized animals and monoclonal antibodies with high binding affinity have been obtained [ 20- 24].

Interestingly, after the desensitization therapy, K1 and b1 values changed to 4.37 × 10−4 (1/10−8 M) and 424 (10−8 M), respectively, indicating that the binding affinity had been attenuated further.

Different chemical modifications to increase nuclease stability and improve binding affinity have been used, for example, in the form of PNA and various LNA-based chemistries.

Linker effects on binding affinity have been seen in other members of this protein family, and we suspect that contributions of the interdomain linker to RNA binding may be a fairly general method of increasing the binding affinity of this family of proteins for RNA.