Sentence examples for bands were stained using from inspiring English sources

Protein bands were stained using Coomassie blue R250.

Finally, the bands were stained using BCIP/NBT solution (Beijing CWBIO Co., Ltd).

After blotting, the filters were washed three times in TBS with 0.1% Tween (TBS-T, pH 7.4), and bands were stained using antibodies listed below, followed by incubation with HRP labeled secondary antibodies.

The respective S-MB and MB bands were stained using Coomassie blue and silver to enhance the sample contrast as described previously [56], and bands corresponding to monomeric and oligomeric peptide were identified using tracking molecular weight markers (MW range 2.5 16.9 kDa, Invitrogen, Carlsbad, CA).

The immunoreactive bands were stained using a light emitting nonradioactive method (ECL; Millipore).

Harvested cells were stained using a standard G-banding technique.

The gels were stained using the SilverQuest silver staining kit (Invitrogen), and the protein bands around 30 75 kDa were excised from the gels.

Microtubules were stained using antibodies.

Nuclei were stained using TOPRO3.

(C ) Vps33 binding to SNARE complex with or without Sec17 was assayed as in A and B, except that Vps33 concentration was varied and protein bands were stained and quantified using SYPRO Ruby.

To do this, purified protein samples were run on SDS-PAGE, transferred to nitrocellulose membranes, protein bands were stained with Ponceau S and excised using a sterile scalpel.