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After thorough washing, the positive bands were revealed using ECL western blotting detection reagents and autoradiography film (Amersham, Biosciences, UK).
Finally, IκB bands were revealed using the ECL™ detection system (Amersham Pharmacia Biotech, Les Ullis, France) according to the manufacturers' instruction.
Protein bands were revealed using enhanced chemiluminescence kit (ECL, Amersham).
Protein bands were revealed using enhanced chemiluminescence (ECL commercial kit).
Specific bands were revealed using a mouse monoclonal anti-thymidylate synthase (TS) antibody (Histoline Laboratories, Italy).
Immunoreactive bands were revealed using an ECL detection kit (GE Healthcare Europe GmbH, Little Chalfont, UK).
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A horseradish conjugated secondary antibody (labeled polymer-HRP antirabbit, Envision system DAKO Cytomation, Carpinteria, CA, USA) was incubated for 45 minutes at room temperature and the corresponding band was revealed using the enhanced chemoluminescence method (Amersham, UK).
Specific bands were detected with the chemiluminescence SuperSignal system (Thermo, Rockford, IL, USA) and were revealed using autoradiographic films.
Images were revealed using ECL plus (Amersham).
After secondary antibody labeling, peroxidase activity was revealed using the western Lightning Plus-ECL kit (Perkin Elmer) and band intensity was quantified using a Kodak Imager.
Signal was revealed using 3,3' diaminobenzidine (DAB).
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