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The positive protein bands were developed using a chemiluminescence system, and the bands were scanned and quantified by densitometric analysis using an image analyzer Quantity One System (Bio-Rad, Richmond, CA, USA).
Bands were developed using the alkaline phosphate substrate BCIP/NBT kit (Thermo Fisher Scientific).
Bands were developed using enhanced chemiluminescence.
The bands were developed using Chemi-Lumi One L (Nacalai Tesque, Kyoto, Japan).
Bands were developed using an ECL Plus detection system (Amersham, Piscataway, NJ).
Inmunoreactive bands were developed using a chemiluminescent substrate (CSPD, Tropix) and Hyperfilm ECL plus films (Amersham, Arlington, Heights, IL).
Similar(36)
The band was developed using BCIP/NBT alkaline phosphatase substrate (Merck Millipore, Darmstadt, Germany).
After washing, the color of a band was developed using FastRed TR/Naphtol AS-MX tablet set.
Band expressions were developed using Pierce ECL reagents (Thermo Scientific, Rockford, IL) and the band densities were quantitated using ImageJ analysis (National Institute of Health, Bethesda, MD).
Protein bands of interest were developed using the ECL chemiluminescence system (Amersham, Buckinghamshire, UK).
Protein bands of interest were developed using an ECL chemiluminescence system (Amersham, Buckinghamshire, UK).
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