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Positive bands were purified using QIAquick Gel extraction kit (Qiagen™, Germany), according to the kit instructions.
Positive bands were purified using Microcon 50 (Billerica, MA) and sequenced using the BigDye Terminator system (Applied Biosystems Inc. [ABI], Foster City, CA).
In case of multiple PCR bands, target bands were purified using a Zymoclean Gel DNA Recovery Kit (Zymo Research Corp., Orange, CA) following the manufacturer's instructions.
Bands were purified using the QIAquick Gel Extraction Kit (Qiagen) and sequenced using the BigDye Terminator cycle sequencing kit (Applied Biosystems, Foster City, CA).
In some samples, PCR bands were purified using a MiniElute PCR Purification Kit (Qiagen, Valencia, CA, USA) and then sequenced to prove that the amplified products corresponded to previously published sequences.
These bands were purified using GFX PCR DNA Purification Kit, Amersham Biosciences.
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PCR runs resulting in a single band were purified using Microcon® YM-30 columns (Millipore) for subsequent sequence analysis.
The resulting amplicon was analyzed on a 1% agarose gel and the specific band was purified using QIA quick Gel Extraction kit (QIAGEN, cat. number 28706).
The second-round PCR products were separated in a 2% agarose gel and the target band was purified using TIANGEN Gel Extracting Kit TIANGEN Inc.. Beijing China).
When two or more amplicons were visualized on a gel, each band was purified using a GFX kit (GE Healthcare, UK), and each fragment was sequenced independently.
The PCR reaction yielded single bands that were purified using PCR Purification Kit (Qiagen).
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