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Bands were analyzed using Gel Pro Analyzer Software (Media Cybernetics) and normalized in respect to corresponding β-actin band and expressed as fold of control [ 23].
The immunoreactive bands were analyzed using a computer-based densitometry Gel-Pro Analyzer (version 6.0, Media Cybernetics, Inc. USA).
Protein bands were analyzed using ImageJ software (National Institutes of Health, USA).
Cell lysates and culture media were collected, PAUF and GAPDH were detected by Western blot (D), and the bands were analyzed using ImageJ (E).
Blots were scanned using the Typhoon scanner (GE Healthcare), and bands were analyzed using ImageQuant™ TL software.
The PCR product was electrophoresed on a 2% TAB agarose gel for 1 hour at 70V and the bands were analyzed using a UV transilluminator.
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The membranes were then scanned on a Bio-Rad Versa DocTM Imaging System (Model 4000) and the intensity of the protein bands was analyzed using the Bio-Rad Quantity One software (Version 4.5.1).
The intensity of the bands was analyzed using Image J (NIH).
The density of the bands was analyzed using the GeneSnap and Gene Tools software (Syngene, Cambridge, UK).
Relative optical density of protein bands was analyzed using gel software image lab 3.0.
The intensity of the bands was analyzed using ImageQuant™ TL (GE Healthcare).
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