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M molecular weight markers.

Molecular weight markers are shown (lane MW).

Lane M represents molecular weight markers.

Lane 1, protein molecular weight markers; lane 2, purified RBUP.

Protein molecular weight markers were purchased from GeneI, Bangalore, India.

Molecular weight markers (kDa) are indicated on the left.

Lane M protein molecular weight markers; lane 1 purified monoclonal antibody 3B2.

The protein bands were compared with P7712S molecular weight markers (New England Biolabs, Ipswich, USA).

Precision Plus Protein Unstained Standards Bio-Radd Laboratories) was used as molecular weight markers.

However, molecular weight markers directly detectable for general use in such systems are not available.

Dual color prestained Precision Plus protein standards (Bio-Rad Laboratories, Richmond, CA, USA) were used as molecular weight markers.

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