The size of the fragments was estimated by comparing with two size markers: Lambda Ladder PFG Marker and MidRange Marker I (New England Biolabs, Beverly, USA).
Lane M, protein size markers.
Molecular size markers were λ DNA/Hind III fragments.
The positions of 8.5 and 12 kDa of size markers are indicated by black bars.
The size markers in the corresponding agarose gel are shown at the left.
The size markers in the RNA ladder on the left are in bp.
The molecular masses (kDa) of the size markers are indicated on the left.
M = DNA size markers, RP = recipient parent, DP = donor parent, H = F1 heterozygote, 1-18 BC1F1 plants.
The gel size was determined using standard molecular weight size markers after the bands were detected by silver staining.
A PCR negative control (no DNA) and size markers (λ/HIII) are shown in the two leftmost lanes.
Template genomic DNA was extracted from a pABr3-transformed mycelium (T) and from a mock-transformed mycelium (WT); size markers (M) are in the leftmost lane.
