Lane 2, molecular mass markers.
The migration of the molecular mass markers is indicated on the left.
This process isothermally amplifies short DNA fragments, called mass markers, to be analyzed by mass spectrometry.
a Plasmid profiling of Escherichia spp. (i), Enterobacter spp. (ii) and Klebsiella spp. (iii) analyzed against V517 molecular mass markers followed by (b).
Lane P1, P2 and P2 PCR products from the plasmid of E. coli JM109 containing dhaD genes; lane C1 negative control (no plasmid); lane M molecular mass markers.
The molecular mass markers for SDS-PAGE were rabbit muscle phosphorylase b (97.0 kDa), bovine serum albumin (66.0 kDa), egg white ovalbumin (45.0 kDa), bovine carbonic anhydrase (30.0 kDa), and trypsin inhibitor (20.1 kDa) purchased from Amersham Biosciences (Uppsala, Sweden).
By varying the sizes of the mass markers, we successfully demonstrated the multiplex SNP genotyping capability of this method by reliably identifying several BRCA mutations in a multiplex manner with mass spectrometry.
Rainbow prestained molecular mass markers (Invitrogen) were used as the standard.
Soybean trypsin inhibitor (20 kDa) and carboanhydrase (29 kDa) were used as molecular mass markers.
The Precision Plus Protein standards were used as molecular mass markers Bio-Rad Laboratoriess, Richmond, CA).
For comparison of molecular mass of Lig proteins, the gel filtration column was calibrated with standard protein molecular mass markers.
