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Exact(25)
Duplicate slide staining with primary antibody omitted was routinely performed to provide a negative control.
DAB (3,3-diaminobenzidine) staining was performed using ultravision mouse tissue detection system kit (labvision) following IHC slide staining DAB LABVISION PROTOCOL.
Two post-hybridisation automatic washes followed by six manual washes to a final stringency of 0.1× SSC (EasyDipTM Slide staining system; Canemco Inc., Quebec, Canada) were performed before scanning.
Male fertility was estimated by extracting mature pollen onto a microscope slide, staining with 2% acetocarmine, and counting the number of full-sized, fully stained grains in a minimum sample of 300 grains (200x magnification).
Immunohistochemistry was performed using the avidin-biotin complex immunostaining method and the automated immunohistochemistry slide staining system by Ventana NexES (Ventana Medical System, Strasbourg, France) as described previously [ 36].
Two post-hybridization automatic washes followed by six manual washes to a final stringency of 0.1× SSC (EasyDipTM Slide staining system; Canemco Inc., Quebec, Canada) were performed before scanning.
Similar(35)
Staining shows little variation in comparison to a slide stained soon after sectioning.
One slide stained for cytokeratin and one negative control slide were analyzed per patient.
A differential cell count was performed using slides stained with the May-Grünwald-Giemsa method.
The samples were generated from breast tissue biopsy slides, stained with hematoxylin and eosin.
Slides stained without the addition of a primary antibody served as negative controls.
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