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Secondly, these could be cells that clumped together or formed interlaced chains which, according to Hayouni et al. [ 33], may decrease staining accuracy.
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Most HPV-positive sperm showed decreased staining with DAPI, indicative of reduced content of DNA.
AP caused decreased staining intensity and aggregation of claudin-1 within the cytosol.
Similarly, Binning et al. report in increased staining for the proliferation marker, Ki67, and decreased staining for cleaved caspase-3 in Shh + HGF-driven mouse medulloblastomas.
Tg+ mice developed progressively decreased staining with SMI-31 as they developed weakness and paralysis reflecting axonal damage and loss with onset of paralysis (Fig. 7B and C).
During development of vasculopathy, signs of arteriolitis were visible by edema and detachment of endothelial cells, decreased staining for αSMA, luminal narrowing, and changes in morphological structure of vascular wall (Figure 1B, C).
Immunofluorescence staining in bones from As2O3-treated rats displayed decreased staining for Runx2 and increased staining for phosphorylated ERK.
We observed decreased staining in SKMEL-5 cells from 3.3 ± 0.69% to 58.1 ± 6.5%, which indicated CGE decreased the mitochondrial membrane potential.
Specifically, skin fibroblasts from healthy old individuals 81-966 yearshowhow decreased staining for HP1 and H3K9me3 compared to the same cells from young individuals (3-11 years) [ 64].
The lungs of BLM-treated CCR7-/ mice, analyzed at day 21, exhibited decreased staining for collagen and retention of the integrity of the alveolar space.
Total, immature CD27+ CD11b− and intermediate-mature CD27+ CD11b+ NK cells in the bone marrow of aged mice had decreased staining with anti-NKG2A/C/E (Fig. 3A), which under normal conditions, mostly stains inhibitory NKG2A in NK cells (Vance et al., 1999).
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