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ISH using a cyclin D1 riboprobe on retinal cryosections from E12.5, E16.5 and P0 revealed strong staining in the ONBL, and diminished staining in the inner neuroblastic layer (INBL), where most postmitotic neurons are located (Figure 2B D).
Quantification of the fluorescent signal area confirmed diminished staining intensity of MT1/2 in diabetic and TETA-treated diabetic LV as compared to control.
The vector-infected and TG2-R580A cellshowededistinctct membranous staining for E-cadherin and β-catenin and diminished staining for fibronectin.
These include: (1) impaired caudal migration of neurons comprising the VIIth motor nucleus and a loss of motor innervation to the face; (2) diminished staining of cholinergic nerves within the palate and retro-orbital fissure; and (3) decreased motor innervation of the tongue by the XIIth nerve.
Furthermore, Cobra1-knockdown cells displayed diminished staining for alkaline phosphatase (AP), an established marker for ESC (Fig. 3G 3I).
In all cell pairs with diminished staining of endogenous mDia1 as a result of mDia1 suppression by RNAi, tangential AJs did not assemble.
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The clearest pSer9GSK3β staining was in the superficial layers of the cortex, with diminishing staining in the deeper layers.
Finally, a chronic Western diet elicited sporadic HCC formation in PEDF KO mice, while the human HCC specimens demonstrated diminished PEDF staining.
Note that apoptotic cells had brighter green staining than normal cells, as well as diminished red staining, and that the green staining was entirely due to JC-1, not Sytox green, as shown by comparing Figure 2C and D. Similar results were obtained with another apoptosis model, Ramos B-lymphoma cells treated overnight with 40 n M paclitaxel.
There was markedly diminished immunohistochemical staining of fibronectin in pressure ulcers.
Splenic cells obtained from BCG-infected mice were treated with exosomes and as shown in figure 1B, CD4+ and CD8+ T cells showed diminished CFSE staining upon treatment with exosomes from infected but not from uninfected J774 cells.
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CEO of Professional Science Editing for Scientists @ prosciediting.com