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Cells located in the subgranular zone between dentate gyrus and hilus show clearly reduced staining in line with reduced hmC levels (Figure 6).
Stage 4 moesin-stained PCa had a significantly reduced staining intensity compared to Stage 2 (p = 0.003).
The DANA and Tamiflu treatments also reduced staining for TGF-β1 (Fig. 7).
N210K (human N195K) and R401K (human R386K) showed nuclear aggregates and reduced staining in the nuclear periphery.
The protective tissue was also maintained throughout staining, which prevented recirculation of dye at the dural surface in this protected area, and further reduced staining quality.
These results were also supported by NAMPT immunohistochemistry. Indeed, we found massive staining of arthritic paw and knee joints from CIA (Fig. 1c), but markedly reduced staining in non-arthritic joints or joints from naïve mice (results not shown).
Significantly reduced staining for Kir4.1 and aquaporin-4 was found mainly in Müller cell endfeet, whereas alterations in the other parts of the cells were not significant (Fig. 2M,N).
Consequently, reduced staining with this dye indicates a decrease in ΔΨ.
HCA treatment of cells also reduced staining with Oil red O, indicative of low triglyceride content.
SaOS-2 osteosarcoma cells treated with PEDF exhibited reduced staining for Ki-67.
Knockdown of Rack1 expression reduced staining both in fat body and midgut cells (Fig. 3A and B).
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