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The presence of layer around the nanoparticle core was due to NKCT1, which was observed by uranyl acetate staining.
Nanostructures were characterized using TEM with uranyl acetate staining.
In contrast, expression of the mCherry-MVP mRNA alone resulted in unstable vaults that rapidly dissociated into halves under conditions used for uranyl acetate staining.
As the intensity of this non-uranyl acetate staining region within AH vaults appeared to vary from vault to vault, we further hypothesized that it is dynamic in nature and could therefore be altered.
Using wide-bore tips, 3 μl of exosome pellet was gently placed on 200-mesh formvar-coated copper grids, allowed to adsorb for 4 5 min, and processed for standard uranyl acetate staining.
When examined by uranyl acetate negatively stained transmission electron microscopy (TEM), AH vaults are morphologically distinct from both native and recombinant MVP-only vaults due to the presence of a significantly large region within the vault lumen that uncharacteristically lacks normal uranyl acetate staining.
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While the absence of dark, uranyl acetate stained areas under TEM is reflective of structural features of the vault (i.
Analyses of the areas stained showed that α-naphthyl acetate stained LNCaP cells approximately three-fold more than RWPE-1 cells.
Electron micrographs of uranyl acetate stained purified membrane fragments show the presence of hemichannel structures whose appearance in these projection images we previously referred to as "doughnut-like".
No doughnut-like structures were seen in these uranyl-acetate stained membranes.
A drop of 1% uranyl acetate stain was added to the grid.
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