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PCR reactions were run in a GeneAmp PCR System 9700 PCR machine.

Quantitative PCR was performed using an ABI-7900 PCR machine.

All PCR reactions were done in a gradient PCR machine (Eppendorf Mastercycler).

Applied Biosystems Step OneTM Real time PCR machine was used to perform qRT-PCR.

PCR was performed in a light cycler Eppendorf PCR machine.

PCR was performed on a Thermal Cycler 170-9703 PCR machine (BIO-RAD, USA).

Real-time PCR was performed on an Applied Biosystems Step One Plus real-time PCR machine.

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Quantitative PCR was performed on a Stratagene RT-PCR machine.

For real-time PCR, the cDNA was amplified using a Stratagene Mx3005P (Stratagene) RT-PCR machine.

Gene expression was quantified using a Step One Plus RT-PCR machine (Applied Biosystems).

Many thanks to Dr. Dawn Luthe for providing access to the Quantitative RT-PCR machine.

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