Sentence examples for pcr instrumentation from inspiring English sources

The robustness and ease of use of the ASFV Invader® assay, combined with the possibility to run and read the assay using simple and relatively inexpensive equipment, makes it suitable for laboratories lacking containment facilities and/or real-time PCR instrumentation or on a regional basis for on-site diagnosis close to putative sites of ASFV outbreaks.

One of the primary barriers to the practicality and availability of NA assays in LRS has been the complexity and power requirements of polymerase chain reaction (PCR) instrumentation (another is sample preparation).

The 2△△ CT method has been used to calculate relative changes in gene expression determined from real-time quantitative PCR experiment [ 47] using the following equations: △△ CT = (CT, miRNA − CT, U6 Salt-stressed broccoli − (CT, miRNA − CT, U6 Salt-stressedcoli, where, the CT values were directly provided from real-time PCR instrumentation.

Real-time reverse-transcriptase PCR (RT-PCR) was performed using LightCycler instrumentation (Roche, Mannheim, Germany) with QuantiTect SYBR green PCR Master Mix (Qiagen, Hilden, Germany) as previously described (Fritsche et al. 2007).

Overall, the assay affords a simple, low-cost, and high-throughput method of BRCA analysis that would be readily accessible to virtually any laboratory with rudimentary instrumentation (PCR machine and basic ELISA plate reader).

The performance capabilities and ease-of-use of TaqMan based real-time PCR chemistries and instrumentation has led to widespread use of this technology as a preferred method for quantifying gene expression as well as for independent validation of microarray results [ 3, 12, 16, 17].

The approach that we describe, which includes the use of an optimized qPCR assay termed quantitative functional index (QFI -PCR, leverages broadly available instrumentation (that is, a real-time thermal cycler) and QFI -PCRes the absoleverageser of amplifiabroadlyplavailable FFPE DNA sample.

It has taken almost twenty-five years from the first research reports of the polymerase chain reaction [25] until recent regulatory clearances have enabled introduction specific PCR- and RT-PCR-based diagnostic assays and instrumentation into clinical practice.

Traditional methods including polymerase chain reaction (PCR) and electrophoresis requires complicated instrumentation and critical environment.

We thank Victor Albert for the use of his facilities and instrumentation for qRT-PCR.

The technology is readily accessible to virtually any laboratory, with the only major instrumentation required being a PCR thermocycler and a basic micro-well plate reader.