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b PCR amplification of inserted fragments.
PCR amplification was performed as described above.
PCR amplification was done as described above.
PCR amplification and 16S rDNA analysis.
Melt curve stage was added after PCR amplification stage.
Detected by PCR amplification, we obtained 20 positive transgenic plants.
The mixture was emulsified and subjected to PCR amplification.
PCR amplification was performed using AmpliTaqGold (Applied Biosystems).
PCR amplification detected deletions in meridionalis-type mtDNA.
Primers YCsPI and PTA were used in PCR amplification.
Additionally, nonspecific PCR amplification was not detected in any lane.
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