Sentence examples for pcr robot from inspiring English sources

For real-time quantitative PCR, the cDNA was amplified in 25 μl reaction mixtures containing 1 μl cDNA, 12.5 μl SYBR® Green PCR Master Mix, primer pairs (Table 1) and nuclease free PCR grade water (Applied Biosystems) using an automated PCR robot CAS-1200™ CAS-1200™ ResearCorbett ResearchK).

The dinucleotide repeat rs2234670 region was amplified using an ABI-877 Integrated Thermal cycler PCR robot with standard reagents (Applied Biosystems) [ 21].

The TA-repeat region was amplified, with one PCR primer fluorescently labelled, using an ABI-877 Integrated Thermal cycler PCR robot with standard reagents (Applied Biosystems) [ 19].

Both precision and reproducibility were generated using multiple lots of RT-PCR reagents, multiple PCR instruments and multiple Tecan liquid-handling robots.

Reaction mixtures were prepared using a liquid-handling CAS-1200 robot (Corbett Robotics) and contained seven PCR standards and each of the prepared cDNAs.

For MT-PCR performance, a strip tube containing Step 1 multiplexed primers was placed in a thermal cycler, along with a Gene-Disc containing lyophilised Step 2 reagents and oil (for covering PCR reactions) on the robot deck.

We used a programmable laboratory robot (TECAN Genesis) augmented with a PCR machine (Biometra TRobot) to perform the liquid handling for PCR, the PCR itself, and the sample preparation for the capillary and gel electrophoresis.

Q-PCR experiments were assembled by a liquid handling robot, a CAS-1200 robot (Corbett Robotics, Australia).

The combination of automated sample preparation using the MagNA Pure extraction robot and the LightCycler PCR system (both Roche Applied Science, Mannheim, Germany) now provides such a standardized application format.

Eight colonies per ORF were analysed for the presence of the ORF of expected size in a colony PCR, utilizing the Perkin Elmer Multiprobe robot to set-up the reactions.

The reactions containing the SensiMix SybrGreen amplification system (Quantace) were set up with a Cas-1200 pipetting robot and analyzed by quantitative PCR on a Rotor-Gene-3000 (Corbett, life science).