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By reintroduction of functional RRN10 gene into the suppressor mutants, we succeeded in constructing a mutant exhibiting higher RNA content than wild type (Chuwattanakul et al. 2011).
Of these mutants, we succeeded in classifying srs3, Srs5, d1, and d61 into cell elongation- or division-type mutants by comparing the cell length and cell number of the inner-epidermal cells of lemma (Izawa et al. 2010; Kitagawa et al. 2010; Segami et al. 2012).
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It proved difficult to establish stable DT40 cell lines expressing rat IP3R1 in which critical residues within the 1-8-14 1-8-14 1-8-14tated, but we succeeded with two motifts.
Through screening of about 1 × 104 Escherichia coli BL21 DE3) transformants, we succeeded in isolating five mutants that showed a 35 217% increase in the secretion level of CGTase-HlyAs61 relative to the wild-type strain.
By replacing these residues through site-directed mutagenesis either with energetically favorable residues or substitutions that do not occur in natural bacterial outer membrane proteins, we succeeded in engineering OmpF mutants with dimeric and monomeric oligomerization states instead of a trimeric oligomerization state.
Finally, in a later stage of the study, we succeeded in isolating an equivalent mutant from the Columbia background [ 32] which had the same properties as those described here for lut2.1.
This time, we succeeded.
"Together we succeeded.
We succeeded.
We succeeded, thrived, excelled; we smashed it.
I think that's how we succeeded".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com