To map the mutation, we intercrossed the mutant that was generated in a C57BL6 background with C3H mice to generate C57BL6 C3H hybrid offspring.
To investigate the relationship between Gli3 and Tulp3 genetically, we intercrossed hitchhiker mutants with mice heterozygous for the Gli3 null allele, Gli3 XtJ (65).
As this early stage of stem cell differentiation was associated with the onset of lethal developmental defects in Dido3 mutants, we established ES cell lines from blastocyst outgrowths obtained from intercrosses of Dido3ΔCT-RFP heterozygotes and analyzed their capacity to proliferate and differentiate in vitro.
The study by Changolkar et al. was performed using a fixed mutation on the C57Bl6 background whereas the mutants we analysed had a genetic background composed of half 129Ola and half C57Bl6 (intercrosses of the F2 generation).
To do so, we intercrossed mice heterozygous for a Blimp1 mutant allele (Ohinata et al., 2005) and retrieved 8-cell stage embryos.
To investigate the consequences of complete loss of miR-1, we intercrossed miR-1-1 and miR-1-2 micent mice (Zhao et al., 2007) to generate double-heterozygous mice in a 129/BL6 mixed background.
We intercrossed the previously described H-2Kb-tsA58 transgenic mouse (Immortomouse), which expresses a temperature-sensitive mutant of the simian virus-40 large T-antigen (tsTAg), with mice of differing Smad genotypes.
To generate homozygous offspring for the rescue experiments, we intercrossed Gad1 or Viaat heterozygotes.
For this purpose we intercrossed RBP-JK5 mice with G-CSF-/ mice to generate RBP-JK5/G-CSF+/− mice.
To generate Asah1P361R/P361R mice, we intercrossed heterozygotes.
