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A library containing scFv mutants was created by error-prone PCR, displayed on the surface of yeast, and higher affinity clones selected by fluorescence activated cell sorting.

A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in ∼50% of the sequence.

A series of mutants was created to disrupt this aromatic cluster formation and study the loss of stability and function under given conditions.

To investigate the importance of these negatively charged residues in the N-terminal region of EvpC, a series of EvpC single, double and triple site-directed mutants was created by substituting D4, E15 and E26 with Ala (Table 2).

Each of these mutants was created in the context of a processed SUMO, which ended with the di-glycine motif at its carboxyl-terminus, and either a six-histidine tag or monomeric Cherry fusion at the amino-terminus.

The library of Pcpt mutants was created using the GeneMorph II Random Mutagenesis Kit (Agilent).

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Nine single cysteine TmArgBP mutants were created and labeled with three different environmentally sensitive fluorescent probes.

Seven CYP24A1 mutants were created and studied by mammalian cell transfection and CYP24A1 activity assay.

For six most frequently occurring mutations, the corresponding single mutants were created to dissect the effects of these mutations.

Mutants are created from the application of syntactic transformation rules (mutation operators) in the code according to the programming language used.

Moreover, T. atroviride mutants were created by mutagenesis using N-methyl-N'-nitro-N-nitrosoguanidine (NTG) as well as UV-light.

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